Dr. Aftab Bashir
Faculty
EXT | 548
aftabbashir@fccollege.edu.pk

KAUSER ABDULLA MALIK SCHOOL OF LIFE SCIENCES

Dr. Aftab has over 30 years of teaching and research experience in various institutions in Pakistan; member Biological Sciences faculty at FCCU since January 2014. He has served as Principal Scientist and Head, Agricultural Biotechnology Division, NIBGE, Faisalabad, faculty member Quaid-e-Azam University and PIEAS, NIBGE campus, Assistant Scientific Officer, Soil Biology Division, NIAB, Faisalabad, supervised research in the areas of cloning and characterization of cellulase genes, isolation and identification of cotton leaf curl virus (CLCuV) genomic components, generation of antibodies against the CLCuV coded proteins, DNA sequencing on ABI 310 and 3100 platforms, expression profiling (qRT-PCR) of cotton fiber development specific genes, isolation, identification and utilization of fiber development specific genes for improving cotton fiber traits (cDNA libraries, ESTs and transcriptome analysis), development of Dipsticks for Cry1Aa and Cry2Ab expression in cotton plants, identification and characterization of novel promoters, synthetic gene designing and construction, development of plant expression vectors, wheat transformation, apex and embryo based transformation in elite cotton and applications of bioinformatics in biological sciences

Research interests: The key focus is on wheat for improving yield and developing resistance against biotic and abiotic stresses using transgenic technologies. Genomics, Transcriptomics and Bioinformatics based designing of expression vectors are the key strengths.

Publications:

International:

  • Bashir, A., Auger, J. A. and Rayburn, A. L. (1993). Flow cytometric DNA analysis of wheat-rye addition lines. Cytometry 14(8): 843-847.
  • Bashir, A., Biradar, D.P. and Rayburn, A.L. (1995). Determining relative abundance of specific DNA sequences in flow cytometrically sorted maize nuclei. Exp. Bot. 46(285): 451-457.
  • Rayburn, A.L., Bashir, A. and Biradar, D.P. (1997). Chromatin composition in Maize F1 hybrids. Maydica 42 (4): 393-399.
  • Rajoka, M. I., Bashir, A. and Malik, K.A. (1997) Mutagenesis of Cellulomonas biazotea for enhanced production of xylanases. Technol. 62 (3):99-108.
  • Mansoor, S., Khan, S.H., Bashir, A., Saeed, M., Zafar, Y. and Malik, K.A. (1997). Evidence for the association of a bipartite geminivirus with tomato leaf curl disease in Pakistan. Plant Dis. 81(8):958-958.
  • Rajoka, M.I., Bashir, A., Hussain, M.R.A., Parvez, S., Ghauri, M.T. and Malik, K.A. (1998). Cloning and expression of β-Glucosidase genes in Escherichia coli and Saccharomyces cerevisiae using Shuttle Vector pYES 2.0. Folia Microbiol. 43(2):129-135.
  • Rajoka, M.I., Bashir, A. (1998). Cloning of multiple Egl genes conferring production of endoglucanase on Escherichia coli. Pakistan Journal of Biological Sciences1(3): 179-182. DOI: 10.3923/pjbs.1998.179.182
  • Rajoka, M. I., Bashir, A., Hussain, S.R.S. and Malik, K.A. (1998). γ-Ray induced mutagenesis of Cellulomonas biazotea for improved production of cellulases. Folia Microbiol. 43 (1): 15-22 .
  • Mansoor, S., Hussain. M., Khan, S.H., Bashir, A., Leghari, A.B., Panwar, G.A., Siddiqui, W.A., Zafar, Y., and Malik, K. A. Polymerase Chain Reaction-based detection of cotton leaf curl and other whitefly-transmitted Geminiviruses from Sindh. Pakistan Journal of Biological Sciences1(1): 39-43. DOI: 10.3923/pjbs.1998.39.43
  • Mansoor, S., Khan, S.H., Bashir, A., Saeed, M., Zafar, Y., Briddon, R., Stanley, J., Markham, P. G. and Malik, K. A. (1999). Identification of a novel circular single-stranded DNA associated with cotton leaf curl disease in Pakistan. Virology 259 (1):190-199.
  • Mansoor, S., Briddon, R.W., Bull, S.E., Bedford, I.D., Bashir, A., Hussain, M., Saeed, M., Zafar, Y., Malik, K.A., Fauquet, C., and Markham, P.G. (2003). Cotton leaf curl disease is associated with multiple monopartite begomoviruses supported by single DNA. Archives of Virology 148:1969-1986.
  • Asad, S., Harris, W.A.A., Bashir, A., Zafar, Y., Malik, K.A., Malik, N.N. and Lichtenstein. C.P. (2003). Transgenic tobacco expressing geminiviral RNAs are resistant to serious viral pathogen causing cotton leaf curl disease. Archives of Virology 148:2341-2352.
  • Rad, M.E., Azarnoosh. M., Bashir, A. and Bakayev, A.A. (2004). A novel vector for expression/secretion of properly folded eukaryotic proteins: a comparative study on cytoplasmic and periplasmic expression of human epidermal growth factor in coli. Iranian Biomedical Journal 8(2):51-61.
  • Iqbal, S., Bashir, A., Naseer, H.M., Ahmed, M. and Malik. K.A. (2008). Identification of differentially expressed genes in developing cotton fibers (Gossypium hirsutum L) through Differential Display. Electronic Journal of Biotechnology [online]. Vol.11 No. 4, January 15, 2008, http://www.ejbiotechnology.info/content/ vol11/issue1/full/11/11.pdf.
  • Naseer, H.M., Iqbal, N., Khatoon A., Bashir A., Zafar, Y. and Malik, K. A. (2010). Molecular characterization and transcriptome profiling of EXPANSIN genes isolated from Calotropis procera Electronic Journal of Biotechnology [online]. Vol.13 No. 5, September 15, 2010, http://www.ejbiotechnology.cl/content/vol13/issue6/full/7/7.pdf
  • Andleeb, S., Amin, I., Bashir, A., Briddon, R. W. and Mansoor, S. (2010). Transient expression of betaC1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions. Virology Journal, 7:373-384.
  • Azhar, M.T., Amin, I., Bashir, A. and Mansoor S. (2011). Characterization of resistance gene analogs from Gossypium arboreum and their evolutionary relationships with homologs from tetraploid cottons. Euphytica. 178 (3): 351-362.
  • Ahmed, M., Bashir, A., Iqbal, S. and Farhatullah (2011). Isolation and identification of differentially expressed genes between Gossypium arboreum and Gossypium hirsutum African Journal of Biotechnology; 10(3): 337-343, 17 January, 2011.
  • Siddique, , Bashir, A. and Latif, F. (2012). Exploration of unique relation among industrial fungi by statistical analysis. Songklanakarin J. Sci. Technol. 34 (6): 1-7.
  • Aslam, U., Khatoon, A., Cheema, H.M.N. and Bashir, A. (2013). Identification and characterization of plasma membrane aquaporins isolated from fiber cells of Calotropis procera. Journal of Zheijang University SCIENCE-B. 14(7):586-595.
  •  Bajwa, K.S., Shahid, A.A., Rao, A.Q., Kiani, M.S., Ashraf, M.A., Dahab, A. A., Bakhsh, A., Latif, A., Azmat, M., Khan, U., Puspito, A.N., Aftab A., Bashir, A. and Hussnain, T. (2013). Expression of Calotropis procera expansin gene CpEXPA3 enhances cotton fiber strength. Australian Journal of Crop Science. 7(2):206-212.
  • Mubeen, H., Bashir, A., Shoaib, M.W., Raza, S. and Hussain, N. (2015). Cloning and characterization of Beta-tubulin gene promoter from Brassica rapa and its expression analysis. International Journal of Scientific and Engineering Research. 6(6):1447-1454. ISSN 2229-5518.
  • Bajwa, K.S., Shahid, A.A., Rao, A. Q., Bashir, A., Aftab, A. and Husnain, T. (2015). Stable transformation and expression of GhEXPA8 fiber expansin gene to improve fiber length and micronaire value in cotton. Frontiers in Plant Science 6(838):1-13 frontiersin.org/Journal/Abstract.aspx?s=1277&name=plant_biotechnology&ART_DOI=10.3389/fpls.2015.00838.
  • Abid, N., Khatoon, A., Maqbool, A., Irfan, , Bashir, A., Asif, I., Shahid, M., Saeed, A., Brinch-Pedersen H. and Malik K.A. (2016). Transgenic expression of phytase in wheat endosperm increases bioavailability of iron and zinc in grains. Accepted in Transgenic Research (Springer), Article number TRAG-D-16-00094R2
  • Masood, A., Iqbal, N., Mubeen, H., Naqvi, R.Z., Khatoon, A. and Bashir, A. (2016). Cloning and expression analysis of alcohol dehydrogenase (Adh) hybrid promoter isolated from Zea mays. African J. Biotechnology, 15(42):2384-2393.
  • Abid, N., Khatoon, A., Maqbool, A.,  Irfan, M., Bashir, A., Asif, I., Shahid, M., Saeed, A., Brinch-Pedersen, H. and Malik, K.A. 2017. Transgenic expression of phytase in wheat endosperm increases bioavailability of iron and zinc in grains. Transgenic Res 26:109–122.
  • Naqvi, R.Z., Asif, M., Saeed, M., Asad, S., Khatoon, A., Amin, I., Mukhtar, Z., Bashir, A., and Shahid Mansoor, S. (2017). Development of a Triple Gene Cry1Ac-Cry2Ab-EPSPS Construct and Its Expression in Nicotiana benthamiana for Insect Resistance and Herbicide Tolerance in Plants. Frontiers in Plant Sciences, 8:1-9.

National:

  • Bashir, A., Ashfaq, S.R., Rajoka, M.I. and Malik, K.A. (1994). Cloning of cellulase genes for the utilization of agricultural wastes. Pakistan Journal of Agricutural Research 15(1): 277-285.
  • Mansoor, S., Bashir, A., Khan, S. H., Hussain M., Saeed M., Zafar, Y., Markham, P. G. and Malik, K. A. (1999). Rapid multiplex PCR for the specific detection of two whitefly-transmitted geminivirus species associated with cotton leaf curl disease in Pakistan. J. Bot., Vol. 31(1):115-123.
  • Naseer, H.M., Khatoon A., Bashir A. and Malik, K. A. (2010). Effect of different antimicrobial agents on the fiber development of in vitro cultured cotton ovules. J. Bot., 42(2): 4235-4242.
  • Indrais, E., Cheema, H.M.N., Samad A. and Bashir, A. (2011). Temporal expression analysis and cloning of cotton (Gossypium hirsutum) fiber genes. Int. J. Agric. Biol. 13:89-94.
  • Bacha, S., Khatoon, A., Asif, M., Yuan, J. and Bashir, A. (2015). Identification and analysis of an efficient dicot constitutive promoter from tomato. Pak. J. Bot., 47(3): 1115-1120.
  • Bacha, S., Khatoon, A.,  Asif,  ,  Yuan, J.  and Bashir, A. (2015). Deletion analysis of Susy-Sl promoter for the identification of optimal promoter sequence. Pak. J. Bot., 47(4): 1287-1292.
  • Iqbal, N., Asia Khatoon, A.,  Muhammad Asif, M.  and Bashir, A. (2016). Expression analysis of fiber related genes in cotton (Gossypium hirsutum ) through Real Time PCR. Pak. J. Bot., 48(3): 1099-1106.
  • Iqbal, N., Masood, A., Bashir, A. and Asif, M. (2016). Histochemical GUS expression of cotton SPS promoter in transgenic tobacco. Journal of Advances in Biology & Biotechnology. 8(1): 1-8.
Address
ROOM | 339, Armacost Science Building
Dr. Aftab Bashir
Faculty
EXT | 548
aftabbashir@fccollege.edu.pk

KAUSER ABDULLA MALIK SCHOOL OF LIFE SCIENCES

Dr. Aftab has over 30 years of teaching and research experience in various institutions in Pakistan; member Biological Sciences faculty at FCCU since January 2014. He has served as Principal Scientist and Head, Agricultural Biotechnology Division, NIBGE, Faisalabad, faculty member Quaid-e-Azam University and PIEAS, NIBGE campus, Assistant Scientific Officer, Soil Biology Division, NIAB, Faisalabad, supervised research in the areas of cloning and characterization of cellulase genes, isolation and identification of cotton leaf curl virus (CLCuV) genomic components, generation of antibodies against the CLCuV coded proteins, DNA sequencing on ABI 310 and 3100 platforms, expression profiling (qRT-PCR) of cotton fiber development specific genes, isolation, identification and utilization of fiber development specific genes for improving cotton fiber traits (cDNA libraries, ESTs and transcriptome analysis), development of Dipsticks for Cry1Aa and Cry2Ab expression in cotton plants, identification and characterization of novel promoters, synthetic gene designing and construction, development of plant expression vectors, wheat transformation, apex and embryo based transformation in elite cotton and applications of bioinformatics in biological sciences

Research interests: The key focus is on wheat for improving yield and developing resistance against biotic and abiotic stresses using transgenic technologies. Genomics, Transcriptomics and Bioinformatics based designing of expression vectors are the key strengths.

Publications:

International:

  • Bashir, A., Auger, J. A. and Rayburn, A. L. (1993). Flow cytometric DNA analysis of wheat-rye addition lines. Cytometry 14(8): 843-847.
  • Bashir, A., Biradar, D.P. and Rayburn, A.L. (1995). Determining relative abundance of specific DNA sequences in flow cytometrically sorted maize nuclei. Exp. Bot. 46(285): 451-457.
  • Rayburn, A.L., Bashir, A. and Biradar, D.P. (1997). Chromatin composition in Maize F1 hybrids. Maydica 42 (4): 393-399.
  • Rajoka, M. I., Bashir, A. and Malik, K.A. (1997) Mutagenesis of Cellulomonas biazotea for enhanced production of xylanases. Technol. 62 (3):99-108.
  • Mansoor, S., Khan, S.H., Bashir, A., Saeed, M., Zafar, Y. and Malik, K.A. (1997). Evidence for the association of a bipartite geminivirus with tomato leaf curl disease in Pakistan. Plant Dis. 81(8):958-958.
  • Rajoka, M.I., Bashir, A., Hussain, M.R.A., Parvez, S., Ghauri, M.T. and Malik, K.A. (1998). Cloning and expression of β-Glucosidase genes in Escherichia coli and Saccharomyces cerevisiae using Shuttle Vector pYES 2.0. Folia Microbiol. 43(2):129-135.
  • Rajoka, M.I., Bashir, A. (1998). Cloning of multiple Egl genes conferring production of endoglucanase on Escherichia coli. Pakistan Journal of Biological Sciences1(3): 179-182. DOI: 10.3923/pjbs.1998.179.182
  • Rajoka, M. I., Bashir, A., Hussain, S.R.S. and Malik, K.A. (1998). γ-Ray induced mutagenesis of Cellulomonas biazotea for improved production of cellulases. Folia Microbiol. 43 (1): 15-22 .
  • Mansoor, S., Hussain. M., Khan, S.H., Bashir, A., Leghari, A.B., Panwar, G.A., Siddiqui, W.A., Zafar, Y., and Malik, K. A. Polymerase Chain Reaction-based detection of cotton leaf curl and other whitefly-transmitted Geminiviruses from Sindh. Pakistan Journal of Biological Sciences1(1): 39-43. DOI: 10.3923/pjbs.1998.39.43
  • Mansoor, S., Khan, S.H., Bashir, A., Saeed, M., Zafar, Y., Briddon, R., Stanley, J., Markham, P. G. and Malik, K. A. (1999). Identification of a novel circular single-stranded DNA associated with cotton leaf curl disease in Pakistan. Virology 259 (1):190-199.
  • Mansoor, S., Briddon, R.W., Bull, S.E., Bedford, I.D., Bashir, A., Hussain, M., Saeed, M., Zafar, Y., Malik, K.A., Fauquet, C., and Markham, P.G. (2003). Cotton leaf curl disease is associated with multiple monopartite begomoviruses supported by single DNA. Archives of Virology 148:1969-1986.
  • Asad, S., Harris, W.A.A., Bashir, A., Zafar, Y., Malik, K.A., Malik, N.N. and Lichtenstein. C.P. (2003). Transgenic tobacco expressing geminiviral RNAs are resistant to serious viral pathogen causing cotton leaf curl disease. Archives of Virology 148:2341-2352.
  • Rad, M.E., Azarnoosh. M., Bashir, A. and Bakayev, A.A. (2004). A novel vector for expression/secretion of properly folded eukaryotic proteins: a comparative study on cytoplasmic and periplasmic expression of human epidermal growth factor in coli. Iranian Biomedical Journal 8(2):51-61.
  • Iqbal, S., Bashir, A., Naseer, H.M., Ahmed, M. and Malik. K.A. (2008). Identification of differentially expressed genes in developing cotton fibers (Gossypium hirsutum L) through Differential Display. Electronic Journal of Biotechnology [online]. Vol.11 No. 4, January 15, 2008, http://www.ejbiotechnology.info/content/ vol11/issue1/full/11/11.pdf.
  • Naseer, H.M., Iqbal, N., Khatoon A., Bashir A., Zafar, Y. and Malik, K. A. (2010). Molecular characterization and transcriptome profiling of EXPANSIN genes isolated from Calotropis procera Electronic Journal of Biotechnology [online]. Vol.13 No. 5, September 15, 2010, http://www.ejbiotechnology.cl/content/vol13/issue6/full/7/7.pdf
  • Andleeb, S., Amin, I., Bashir, A., Briddon, R. W. and Mansoor, S. (2010). Transient expression of betaC1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions. Virology Journal, 7:373-384.
  • Azhar, M.T., Amin, I., Bashir, A. and Mansoor S. (2011). Characterization of resistance gene analogs from Gossypium arboreum and their evolutionary relationships with homologs from tetraploid cottons. Euphytica. 178 (3): 351-362.
  • Ahmed, M., Bashir, A., Iqbal, S. and Farhatullah (2011). Isolation and identification of differentially expressed genes between Gossypium arboreum and Gossypium hirsutum African Journal of Biotechnology; 10(3): 337-343, 17 January, 2011.
  • Siddique, , Bashir, A. and Latif, F. (2012). Exploration of unique relation among industrial fungi by statistical analysis. Songklanakarin J. Sci. Technol. 34 (6): 1-7.
  • Aslam, U., Khatoon, A., Cheema, H.M.N. and Bashir, A. (2013). Identification and characterization of plasma membrane aquaporins isolated from fiber cells of Calotropis procera. Journal of Zheijang University SCIENCE-B. 14(7):586-595.
  •  Bajwa, K.S., Shahid, A.A., Rao, A.Q., Kiani, M.S., Ashraf, M.A., Dahab, A. A., Bakhsh, A., Latif, A., Azmat, M., Khan, U., Puspito, A.N., Aftab A., Bashir, A. and Hussnain, T. (2013). Expression of Calotropis procera expansin gene CpEXPA3 enhances cotton fiber strength. Australian Journal of Crop Science. 7(2):206-212.
  • Mubeen, H., Bashir, A., Shoaib, M.W., Raza, S. and Hussain, N. (2015). Cloning and characterization of Beta-tubulin gene promoter from Brassica rapa and its expression analysis. International Journal of Scientific and Engineering Research. 6(6):1447-1454. ISSN 2229-5518.
  • Bajwa, K.S., Shahid, A.A., Rao, A. Q., Bashir, A., Aftab, A. and Husnain, T. (2015). Stable transformation and expression of GhEXPA8 fiber expansin gene to improve fiber length and micronaire value in cotton. Frontiers in Plant Science 6(838):1-13 frontiersin.org/Journal/Abstract.aspx?s=1277&name=plant_biotechnology&ART_DOI=10.3389/fpls.2015.00838.
  • Abid, N., Khatoon, A., Maqbool, A., Irfan, , Bashir, A., Asif, I., Shahid, M., Saeed, A., Brinch-Pedersen H. and Malik K.A. (2016). Transgenic expression of phytase in wheat endosperm increases bioavailability of iron and zinc in grains. Accepted in Transgenic Research (Springer), Article number TRAG-D-16-00094R2
  • Masood, A., Iqbal, N., Mubeen, H., Naqvi, R.Z., Khatoon, A. and Bashir, A. (2016). Cloning and expression analysis of alcohol dehydrogenase (Adh) hybrid promoter isolated from Zea mays. African J. Biotechnology, 15(42):2384-2393.
  • Abid, N., Khatoon, A., Maqbool, A.,  Irfan, M., Bashir, A., Asif, I., Shahid, M., Saeed, A., Brinch-Pedersen, H. and Malik, K.A. 2017. Transgenic expression of phytase in wheat endosperm increases bioavailability of iron and zinc in grains. Transgenic Res 26:109–122.
  • Naqvi, R.Z., Asif, M., Saeed, M., Asad, S., Khatoon, A., Amin, I., Mukhtar, Z., Bashir, A., and Shahid Mansoor, S. (2017). Development of a Triple Gene Cry1Ac-Cry2Ab-EPSPS Construct and Its Expression in Nicotiana benthamiana for Insect Resistance and Herbicide Tolerance in Plants. Frontiers in Plant Sciences, 8:1-9.

National:

  • Bashir, A., Ashfaq, S.R., Rajoka, M.I. and Malik, K.A. (1994). Cloning of cellulase genes for the utilization of agricultural wastes. Pakistan Journal of Agricutural Research 15(1): 277-285.
  • Mansoor, S., Bashir, A., Khan, S. H., Hussain M., Saeed M., Zafar, Y., Markham, P. G. and Malik, K. A. (1999). Rapid multiplex PCR for the specific detection of two whitefly-transmitted geminivirus species associated with cotton leaf curl disease in Pakistan. J. Bot., Vol. 31(1):115-123.
  • Naseer, H.M., Khatoon A., Bashir A. and Malik, K. A. (2010). Effect of different antimicrobial agents on the fiber development of in vitro cultured cotton ovules. J. Bot., 42(2): 4235-4242.
  • Indrais, E., Cheema, H.M.N., Samad A. and Bashir, A. (2011). Temporal expression analysis and cloning of cotton (Gossypium hirsutum) fiber genes. Int. J. Agric. Biol. 13:89-94.
  • Bacha, S., Khatoon, A., Asif, M., Yuan, J. and Bashir, A. (2015). Identification and analysis of an efficient dicot constitutive promoter from tomato. Pak. J. Bot., 47(3): 1115-1120.
  • Bacha, S., Khatoon, A.,  Asif,  ,  Yuan, J.  and Bashir, A. (2015). Deletion analysis of Susy-Sl promoter for the identification of optimal promoter sequence. Pak. J. Bot., 47(4): 1287-1292.
  • Iqbal, N., Asia Khatoon, A.,  Muhammad Asif, M.  and Bashir, A. (2016). Expression analysis of fiber related genes in cotton (Gossypium hirsutum ) through Real Time PCR. Pak. J. Bot., 48(3): 1099-1106.
  • Iqbal, N., Masood, A., Bashir, A. and Asif, M. (2016). Histochemical GUS expression of cotton SPS promoter in transgenic tobacco. Journal of Advances in Biology & Biotechnology. 8(1): 1-8.
Address
ROOM | 339, Armacost Science Building
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